Policies> Mouse Specific Pathogen Free Policy
PURPOSE
This policy is intended to describe which mouse pathogens must be tested for in animals
being introduced to Animal Care Services (ACS) colonies. ACS strives to provide the
highest standards of rodent health care for research investigators. The term specific
pathogen free (SPF) is defined by what pathogens are excluded. The individual pathogens
actually excluded are commonly limited by the health status of the mice available from
the commercial vendors. As commercial vendor colonies eliminate additional agents, the
criteria at the University of Florida (UF) will also be modified. Listed below are the
agents specifically excluded from the UF mouse colonies currently.
2 SCOPE
This policy applies to all mice being introduced to an ACS facility.
3 POLICY
In addition to commercial vendor and ACS sentinel animal pathogen testing, all animals
that Principal Investigators (PI) intend to have brought to the UF must be shown through
adequate testing to be free of the pathogens listed below. PIs must coordinate all animal “importations” from other institutions through the ACS. ACS veterinary involvement
will help to ensure that excluded pathogens are not inadvertently introduced to the UF
colonies.
3.1 Pathogens excluded
3.1.1 VIRUSES
Ectromelia
Epizootic Diarrhea of Infant Mice Virus (EDIM) Rotavirus
Hanta (Hantaan) Virus (HTN)
K Virus
Lactate Dehydrogenase Elevating Virus
Lymphocytic Choriomeningitis Virus (LCMV)
Minute Virus of Mice (MVM) (MMV)
Mouse Adenovirus 1,2 (MAV, MAd)
Mouse CytomegaloVirus (MCMV)
Mouse Hepatitis Virus
Mouse Parvovirus (MPV)
Mouse Thymic Virus (MTV, MTLV) Herpes
Pneumonia Virus of Mice (PVM)
Polyoma Virus
Reovirus 3
Sendai Virus
Theiller’s Murine Encephalomyelitis Virus (GDVII) (TMEV)
3.1.2 BACTERIA AND FUNGI
CAR bacillus
Citrobacter rodentium
Clostridium piliforme
Corynebacterium kutcheri
Helicobacter sp. – Select rooms in the Cancer Genetics Building
Mycoplasma pulmonis
Pasteurella pneumotropica
Salmonella spp.
Streptobacillus moniliformis
3.1.3 PARASITES
Encephalitozoon cuniculi
Fur mites
Pinworms
