Zebrafish offer several benefits to researchers. External fertilization, small size, rapid development, high fecundity (100-200 viable embryos per female per week), and optical transparency of embryos are some of the many advantages that allow for high-throughput, non-invasive in vivo observation and imaging during studies.
ACS maintains a colony of wild-type animals as a resource to investigators. Research specific lines can be imported upon request. In addition, ACS has the capability to generate transgenic and mutant animals in-house using targeted gene technology.
Potential Research Areas
- Toxicological studies
- Role of microbiota in host response
- High-throughput small molecule screens
- Inflammation/immune response
- Gut ecology
- Role of gene function in disease
Imaging and Procedure Capabilities
ACS maintains a Leica S6E Stereomicroscope for basic screening of embryos and for performing genetic manipulations using a Drummond Nanoject II microinjector.
In addition, a Leica M205FA Fully Automated Fluorescence Stereomicroscope, coupled with a Leica DFC365 FX digital camera allows for work with transgenic animals.
Current imaging options
- Single channel and bright field imaging
- Dual fluorescent channel overlay (currently GFP and TxRed only)
- Time-lapse and real-time movies
Additional fluorescent filters can be purchased upon PI request.
For more information on use of the ACS Zebrafish Services, please contact: