Zebrafish Services

 

ACS can maintain both adults (tanks with blue baffles) and larvae/juveniles (half-full tanks with yellow or green baffles) on a single system.

Zebrafish offer several benefits to researchers.  External fertilization, small size, rapid development, high fecundity (100-200 viable embryos per female per week), and optical transparency of embryos are some of the many advantages that allow for high-throughput, non-invasive in vivo observation and imaging during studies. 

Strain Options

ACS maintains a colony of wild-type animals as a resource to investigators.  Research specific lines can be imported upon request.  In addition, ACS has the capability to generate transgenic and mutant animals in-house using targeted gene technology. 

Potential Research Areas

  • Toxicological studies
  • Role of microbiota in host response
  • Physiology
  • High-throughput small molecule screens
  • Nutrition/metabolism
  • Inflammation/immune response
  • Gut ecology
  • Role of gene function in disease

Imaging and Procedure Capabilities

ACS maintains a Leica S6E Stereomicroscope for basic screening of embryos and for performing genetic manipulations using a Drummond Nanoject II microinjector.

In addition, a Leica M205FA Fully Automated Fluorescence Stereomicroscope, coupled with a Leica DFC365 FX  digital camera allows for work with transgenic animals. 

Current imaging options

  • Single channel and bright field imaging
  • Dual fluorescent channel overlay (currently GFP and TxRed only)
  • Time-lapse and real-time movies

Additional fluorescent filters can be purchased upon PI request. 

For more information on use of the ACS Zebrafish Services, please contact:

 Dr. Karl Andrutis at 352-273-9246, kandrutis@ufl.edu or Dr. Harvey Ramirez at 352-273-9243, hramir@ufl.edu