Germ Free Services

Germ Free Mice

Germ Free Mice

Mice are housed in flexible film or semi-rigid isolators in a completely germ free environment. This provides an environment which allows investigators to conduct experiments without competing background levels of microbiota. Investigators can manipulate the microbiota by selectively colonizing germ-free rodents with single or multiple, commensal or pathogenic, bacterial or fungal species. Once colonized, the mice are maintained as gnotobiotics by housing them in sterile isolators to avoid cross contamination with other bacteria or fungi.

Germ-Free mice are appropriate for use in a number of experimental investigations such as:

  • Pathogenesis of infectious diseases
  • The role of microbiota in chronic disease (immune-mediated, genetic, neoplastic, etc)
  • Physiology of the gut
  • The mucosal immune system
  • Nutrition
  • Inflammation and sepsis
  • Oral tolerance and allergic responses
  • Probiotics
  • Ocular, dental, and periodontal disease
  • Dermatitis
  • Gut ecology

Animal Care Services maintains a limited number of breeding colonies germ free mouse strains. Strains of germ free mice maintained at the University of Florida are listed below.

  • WT C57BI/6
  • WT SvEv 129
  • IL-10KO C57BI/6
  • IL-10KO SvEv 129
  • C57BL/6J-ApcMin/J

If you want to know more about this service, availability and associated cost and please contact the ACS Director’s Office at 352-273-9246.