Mouse Models Core

Since January 2016 ACS has produced 385 Transgenic Founders.

 

Click on links below to access service request forms and service descriptions

Transgenic Mice Creation

We will inject your transgene expression construct DNA into zygote pronuclei to produce Transgenic Mice by gene transfer
[Guarantee: 30 live births per transgene construct]

Knockout/Knockin Mice Generation

We will inject your targeted embryonic stem cells into blastocysts to produce Knockout or Knockin Mice
[Guarantee: 40 blastocyst injections per ES cell line]

Sperm Cryopreservation

We will cryopreserve sperm collected from two of your transgenic and/or KO males. Session fee includes one quality control.
[Guarantee: If any quality control fails for a given session, one new session is performed at no extra charge]

Rederivation of mouse lines to eliminate pathogens

We will decontaminate your line by mating your studs to our females, and transferring the resulting embryos or newborn pups to SPF foster mothers. Rederived lines will require isolator quarantine and pathogen testing, in addition to the regular fee.

Reconstitution of cryopreserved embryos

We will thaw your frozen embryos and transfer them into suitable SPF foster mothers. Imported embryos will require isolator quarantine and pathogen testing, in addition to the regular fee.

Reconstitution of cryopreserved sperm

We will thaw your frozen sperm, perform IVFs, and transfer the resulting fertilized eggs into suitable SPF foster mothers. Imported sperm will require isolator quarantine and pathogen testing, in addition to the regular fee.

Pre-implantation Embryo Collection

We will collect pre-implantation embryos and deliver them to you for your experiments, using our standard strains, or your animals.

Transgenic animal registration form

You can contact ACS transgenic services at:  ACS-MMC@acs.ufl.edu